Tuesday, September 26, 2006

CodonCode Aligner Tutorials: EST analysis

CodonCode Aligner Tutorials: EST analysis
CodonCode Aligner Tutorials:

From this page, you will be able to view tutorials for CodonCode Aligner. Currently available tutorial are:

New Features in CodonCode Aligner version 1.6:

New Features in CodonCode Aligner version 1.5:

This Quick Tour will show the most important features of CodonCode Aligner:

The tour is divided into three parts:

  • Part 1: Basic Features. This part shows you Aligner's main functions, and can be done in Demo mode, without requesting a trial license. It demonstrates how to call bases with Phred, trim ends, vector screen, assemble, and edit.
  • Part 2: Importing, Exporting, and Saving. This part first shows how to request a fully functional, time-limited trial license that you need for this second part of the tour. It then shows how to add sequences and assemblies to Aligner projects, how to save Aligner projects, and how to export sequences and entire projects.
  • Part 3: Analyzing Heterozygous Mutations. This part describes how to find and analyze heterozygous mutations in CodonCode Aligner - heterozygous point mutations as well as heterozygous insertions and deletions ("indels").
This quick tour is available online at http://www.codoncode.com/aligner/quicktour/. If you downloaded and installed CodonCode Aligner, a copy of this tour has been installed in your installation directory as an Adobe PDF file named "QuickTour.pdf".

Steps:
Base Calling
Clip Ends
Trim Vector
Assemble with options... / Unassemble existing contigs (assemble from the scratch)
(Algorithm tab: Use PHRAP and Use built-in algorithm)